MTNR1B Antibody
| Code | Size | Price |
|---|
| PSI-42-063-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
MTNR1B, melatonin receptor 1B, MEL-1B-R, MT2, melatonin receptor MEL1B, melatonin receptor type 1B
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave approx .48kDa and 37kDa bands in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_005950.1. The 48kDa and 37kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Cerebellum shows membranous staining primarily in Purkinje cells. Recommended concentration, 3-6ug/ml.
Background References:
- Rawashdeh O, de Borsetti NH, Roman G, Cahill GM. Melatonin suppresses nighttime memory formation in zebrafish. Science 2007 Nov 318 (5853): 1144-6.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QDASKGSHAEGLQSP
NCBI Gene ID #:
4544
NCBI Official Name:
melatonin receptor 1B
NCBI Official Symbol:
MTNR1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005950.1
Protein GI Number:
5174595
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,Neuroscience
Swissprot #:
P49286