S100A4 Antibody

ProSci
Product Code: PSI-46-326
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-326-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-326 (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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46-326 (0.05ug/ml) staining of Peripheral blood mononuclear cells lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Further Information

Additional Names:
S100A4, CAPL, S100 calcium binding protein A4, 18A2, 42A, MTS1, P9KA, PEL98, S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), S100 calcium-binding protein A4, S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), malignant transformation suppression 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 13kDa band observed in Human Peripheral blood mononuclear cells lysates (calculated MW of 11.7kDa according to NP_002952.1). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Small Intestine shows staining of lymphatic cells in the lamina propria. Recommended concentration: 1-2ug/ml.
Background References:
  • Li ZH, Bresnick AR. The S100A4 metastasis factor regulates cellular motility via a direct interaction with myosin-IIA. Cancer Res. 2006 May 15;66(10):5173-80.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: CNEFFEGFPDKQP
NCBI Gene ID #:
6275
NCBI Official Name:
S100 calcium binding protein A4
NCBI Official Symbol:
S100A4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002952.1, NP_062427.1
Protein GI Number:
4506765
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Both variants (NP_002952.1; NP_062427.1) represent identical protein.
Swissprot #:
P26447