PRDM16 Antibody

ProSci
Product Code: PSI-46-216
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-216-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-216 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

46-216 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

Further Information

Additional Names:
PRDM16, MEL1, KIAA1675, PR domain containing 16, transcription factor MEL1, MGC166915, PFM13, MDS1/EVI1-like, PR-domain zinc finger protein 16
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

ChIP: This product has been successfully used in ChIP on Mouse: Hondares E et al. (2011) Cell Transplant.?2011;20(8):1179-92. PMID: 21294954.

Background References:
  • Mochizuki N, Shimizu S, Nagasawa T, Tanaka H, Taniwaki M, Yokota J, Morishita K. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood 2000 Nov 1;96(9):3209-14
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-TSESGAFHPINHL
NCBI Gene ID #:
63976
NCBI Official Name:
PR domain containing 16
NCBI Official Symbol:
PRDM16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071397.3, NP_955533.2
Protein GI Number:
289547573
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms.
Swissprot #:
Q9HAZ2