PTF1a Antibody

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ProSci
Product Code: PSI-46-247
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-247-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-247 (0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-247 (0.5ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
PTF1A, PTF1-p48, pancreas specific transcription factor, 1a, bHLH transcription factor p48, class II bHLH protein PTF1A, exocrine pancreas-specific transcription factor p48, p48 DNA-binding subunit of transcription factor PTF1, pancreas transcription factor 1 subunit alpha
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 35.0kDa according to NP_835455.1). Recommended concentration: 0.5-1.5ug/ml.
Background References:
  • Pascual M, Abasolo I, Mingorance-Le Meur A, Martinez A, Del Rio JA, Wright CV, Real FX, Soriano E. Cerebellar GABAergic progenitors adopt an external granule cell-like phenotype in the absence of Ptf1a transcription factor expression. Proc Natl Acad Sci USA. 2007 Mar 20;104(12):5193-8. Epub 2007 Mar 14.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Zebrafish
Immunogen:
The immunogen for this antibody is: C-WTDEKQLKEQN
NCBI Gene ID #:
256297
NCBI Official Name:
pancreas specific transcription factor, 1a
NCBI Official Symbol:
PTF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_835455.1
Protein GI Number:
30039710
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q7RTS3