ACPP Antibody

ProSci
Product Code: PSI-42-484
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-484-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-484 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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42-484 (0.3ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP, prostatic acid phosphotase
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 Oct;32(10):1832-5. PMID: 3530530). Recommended concentration: 0.3-1ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5ug/ml.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-YGIHKQKEKSRLQ
NCBI Gene ID #:
55
NCBI Official Name:
acid phosphatase, prostate
NCBI Official Symbol:
ACPP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001090.2
Protein GI Number:
6382064
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
This antibody is expected to recognize both isoforms (NP_001090.2; NP_001127666.1).
Swissprot #:
P15309