PDLIM5 Antibody

ProSci
Product Code: PSI-25-451
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-25-451-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDLIM5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
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Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
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Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
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Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
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Antibody used in WB on HepG2 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).

Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in WB on Hum. Fetal Brain at 1 ug/ml.
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Antibody used in WB on HepG2 at 1 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).

Further Information

Additional Names:
PDLIM5, ENH, ENH1, L9, LIM
Application Note:
PDLIM5 antibody can be used for detection of PDLIM5 by ELISA at 1:312500. PDLIM5 antibody can be used for detection of PDLIM5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PDLIM5 is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized.
Background References:
  • Squassina, A., (2008) Psychiatr. Genet. 18 (3), 128-132.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDLIM5.
NCBI Gene ID #:
10611
NCBI Official Name:
PDZ and LIM domain 5
NCBI Official Symbol:
PDLIM5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
NP_006448
Protein GI Number:
374092020
Purification:
Antibody is purified by peptide affinity chromatography method.
Research Area:
Transcription ,Signal Transduction
Swissprot #:
Q96HC4
User NOte:
Optimal dilutions for each application to be determined by the researcher.