SMAD6 Antibody

Contact us
ProSci
Product Code: PSI-27-669
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-27-669-100ul100ul£609.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

1 / 3
Antibody used in IHC on Human Lung.
2 / 3
3 / 3
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.
❮❯

Antibody used in IHC on Human Lung.
Antibody used in WB on Human Jurkat 0.5-1.0 ug/ml.

Documents

Further Information

Additional Names:
SMAD6, AOVD2, MADH6, MADH7, HsT17432
Application Note:
SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:62500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.
Background References:
  • Tsunobuchi, H., et al., (2004) Biophys. Res. Commun. 316(3), 712-719.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6.
NCBI Gene ID #:
4091
NCBI Official Name:
SMAD family member 6
NCBI Official Symbol:
SMAD6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
NP_005576
Protein GI Number:
92859871
Purification:
Antibody is supplied as total IgG.
Research Area:
Transcription ,Signal Transduction
Swissprot #:
O43541
User NOte:
Optimal dilutions for each application to be determined by the researcher.