CCL20 Antibody

ProSci
Product Code: PSI-42-213
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-213-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-213 (6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

42-213 (6ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
CCL20, chemokine (C-C motif) ligand 20, CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38, exodus-1, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A (Cys-Cys), member 20
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments in Human Peripheral Blood Mononucleocytes lysates gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Liver shows local staining between the hepatocytes.. Recommended concentration, 6-8ug/ml.
Background References:
  • Punj V, Matta H, Schamus S, Yang T, Chang Y, Chaudhary PM, Induction of CCL20 production by Kaposi sarcoma-associated herpesvirus: role of viral FLICE inhibitory protein K13-induced NF-kappaB activation. Blood 2009 May 113 (22): 5660-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Cow, Pig
Immunogen:
The immunogen for this antibody is: CLGYTDRILHPK
NCBI Gene ID #:
6364
NCBI Official Name:
chemokine (C-C motif) ligand 20
NCBI Official Symbol:
CCL20
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004582.1, NP_001123518.1
Protein GI Number:
4759076
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Immunology ,Innate Immunity
SPECIFICITY:
This antibody is expected to recognize both isoforms (NP_004582.1; NP_001123518.1).
Swissprot #:
P78556