MC5R Antibody

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ProSci
Product Code: PSI-42-031
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-42-031-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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42-031 (0.3ug/ml) staining of Mouse Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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42-031 (0.3ug/ml) staining of Mouse Muscle lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
MC5R N: melanocortin 5 receptor
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx. 37kDa band observed in Mouse Skeletal Muscle lysates (calculated MW of 36.6kDa according to NP_005904.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptideImmunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 2.5ug/ml.
Background References:
  • Grieco P, Cai M, Han G, Trivedi D, Campiglia P, Novellino E, Hruby VJ. Further structure-activity studies of lactam derivatives of MT-II and SHU-9119: their activity and selectivity at human melanocortin receptors 3, 4, and 5. Peptides. 2007 Jun;28(6):1191-6. Epub 2007 Mar 1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PNVKNKSSPCED
NCBI Gene ID #:
4161
NCBI Official Name:
melanocortin 5 receptor
NCBI Official Symbol:
MC5R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005904.1
Protein GI Number:
5174535
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
SPECIFICITY:
.
Swissprot #:
P33032