MCP-2 Antibody

ProSci
Product Code: PSI-38-118
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-38-118-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
MCP-2 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

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To detect mMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine MCP-2 (38-117) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mMCP-2.
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To detect mMCP-2 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Murine MCP-2 (38-117) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant mMCP-2.

Further Information

Additional Names:
HC14, Mcp2, MCP-2, Scya8, AB023418, 1810063B20Rik, C-C motif chemokine 8, Monocyte chemoattractant protein 2
Application Note:
ELISA:

Sandwich:
To detect mMCP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine MCP-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mMCP-2.

Western Blot:
To detect mMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
MCP 2 is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMCP-2. Murine MCP-2 specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
20307
NCBI Official Name:
chemokine (C-C motif) ligand 8
NCBI Official Symbol:
Ccl8
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
Q9Z121
Protein GI Number:
13431906
Research Area:
Chemokines & Cytokines,Antibody Pairs
Swissprot #:
Q9Z121
User NOte:
Centrifuge vial prior to opening.