HCLS1 Antibody
| Code | Size | Price |
|---|
| PSI-27-647-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCLS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
HCLS1, HS1, CTTNL
Application Note:
HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:2500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.
Background References:
- Ruzzene, M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCLS1.
NCBI Gene ID #:
3059
NCBI Official Name:
hematopoietic cell-specific Lyn substrate 1
NCBI Official Symbol:
HCLS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
NP_005326
Protein GI Number:
167234422
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Signal Transduction ,Immunology
Swissprot #:
P14317
User NOte:
Optimal dilutions for each application to be determined by the researcher.