FES Monoclonal Antibody

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ProSci
Product Code: PSI-32-153
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-153-0.1mL0.1mL£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG1
Antibody Clonality: Monoclonal
Antibody Clone: 5A11G5
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
FES monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.
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Western blot analysis using FES monoclonal antibody against truncated FES - His recombinant protein.

Documents

Further Information

Additional Names:
FPS, Proto-oncogene c-FesFPS
Application Note:
Western Blot:1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
FES (feline sarcoma oncogene) and Fer are the only two members of a unique family of cytoplasmic protein tyrosine kinases.FES and Fer contain a central Src homology-2 (SH2) domain and a carboxy-terminal tyrosine kinase catalytic domain. They are structurally distinguished from other members of cytoplasmic protein tyrosine kinase subfamilies by the presence of amino-terminal Fer/CIP4 homology and coiled-coil domains. FES was originally identified as an oncogene from avian and feline retroviruses. Human c-Fes has been implicated in myeloid, vascular endothelial and neuronal cell differentiation. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. Mutations may activate the FES kinase and thereby contribute to cancer. However, recent data strongly suggests that the c-FES protein-tyrosine kinase is a tumor suppressor rather than a dominant oncogene in colorectal cancer.
Background References:
  • Delfino FJ. Stevenson H. Smithgall TE. J Biol Chem. 2006, Mar 31, 281(13): 8829-35. Epub 2006 Feb 2.
  • Durfee WK. Rivard A. J Biomech Eng. 2005, Nov, 127(6):1014-9.
  • Vitenzon AS. Mironov EM. Petrushanskaya KA. Neurosci Behav Physiol. 2005, Sep, 35(7):709-14.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant FES-His expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
2242
NCBI Official Name:
feline sarcoma oncogene
NCBI Official Symbol:
FES
NCBI Organism:
Homo sapiens
Protein Accession #:
P07332
Protein GI Number:
115502390
Research Area:
Signal Transduction
Swissprot #:
P07332
User NOte:
Optimal dilutions for each application to be determined by the researcher.