WASP Antibody

ProSci
Product Code: PSI-46-591
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-591-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-591 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-591 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Further Information

Additional Names:
WAS, WASP, Wiskott-Aldrich syndrome (eczema-thrombocytopenia), THC, IMD2, thrombocytopenia 1 (X-linked), Wiskott-Aldrich syndrome (eczema-thrombocytopenia) protein, THC1, Wiskott-Aldrich syndrome protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Paraffin embedded Human Spleen. Recommended concentration: 5?g/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HepG2 and U2OS cells. Recommended concentration: 10?g/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Background References:
  • Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J. Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 2005 Jan 31;168(3):441-52.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-SPADKKRSGKKKI
NCBI Gene ID #:
7454
NCBI Official Name:
Wiskott-Aldrich syndrome
NCBI Official Symbol:
WAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000368.1
Protein GI Number:
4507909
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
No cross-reactivity expected with N WASP (WASL).
Swissprot #:
P42768