RSK1 Monoclonal Antibody

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ProSci
Product Code: PSI-32-212
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-32-212-0.1mg0.1mg£560.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: IgG2a
Antibody Clonality: Monoclonal
Antibody Clone: 10B1D7A9
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
RSK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.
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Western blot analysis using RSK1 monoclonal antibody against truncated RSK1 - His recombinant protein.

Documents

Further Information

Additional Names:
MAPKAPK1A, RSK1, 90 kDa ribosomal protein S6 kinase 1, S6K-alpha-1, RSK, HU-1, MAPKAPK1A
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Background:
Rsk1 is a member of a family of 90 kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB. RSK proteins possess two separate kinase domains, the C terminal kinase domain and the N terminal kinase domain, separated by a linker region containing a hydrophobic motif. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation.
Background References:
  • Alcorta, D.A., et al. 1989. Mol. Cell. Biol. 9:3850-3859.
  • Sweet, L.J., et al. 1990. Mol. Cell. Biol. 10: 2413-2417.
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Ni-NTA purified truncated recombinant RSK1 expressed in E. Coli strain BL21 (DE3).
NCBI Gene ID #:
6195
NCBI Official Name:
ribosomal protein S6 kinase, 90kDa, polypeptide 1
NCBI Official Symbol:
RPS6KA1
NCBI Organism:
Homo sapiens
Protein Accession #:
Q15418
Protein GI Number:
20178306
Research Area:
Signal Transduction
Swissprot #:
Q15418
User NOte:
Optimal dilutions for each application to be determined by the researcher.