OTUB1 Antibody

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ProSci
Product Code: PSI-46-881
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-881-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-881 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-881 (0.05ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
OTUB1, OTU domain, ubiquitin aldehyde binding 1, FLJ20113, FLJ40710, HSPC263, MGC111158, MGC4584, OTB1, OTU1, OTU-domain Ubal-binding 1, otubain 1, ubiquitin-specific protease otubain 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx. 30kDa band observed in Mouse Brain lysates (calculated MW of 31.3kDa according to Human NP_060140.2 and Mouse NP_598911.1). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Juris SJ, Shah K, Shokat K, Dixon JE, Vacratsis PO. Identification of otubain 1 as a novel substrate for the Yersinia protein kinase using chemical genetics and mass spectrometry. FEBS Lett. 2006 Jan 9;580(1):179-83.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-YKEYAEDDNIYQQK
NCBI Gene ID #:
55611
NCBI Official Name:
OTU deubiquitinase, ubiquitin aldehyde binding 1
NCBI Official Symbol:
OTUB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060140.2
Protein GI Number:
109148508
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q96FW1