PCK1 Antibody
| Code | Size | Price |
|---|
| PSI-27-714-100ul | 100ul | £609.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Canine (dog)
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PCK1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
PCK1, PEPCK1, PEPCKC, PEPCK-C
Application Note:
PCK1 antibody can be used for detection of PCK1 by ELISA at 1:1562500. PCK1 antibody can be used for detection of PCK1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.
Background References:
- Hajarnis, S., et al., (2005) J Biol Chem. 280(31), 28272-80.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCK1.
NCBI Gene ID #:
5105
NCBI Official Name:
phosphoenolpyruvate carboxykinase 1 (soluble)
NCBI Official Symbol:
PCK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
NP_002582
Protein GI Number:
187281517
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Apoptosis ,Homeostasis
Swissprot #:
P35558
User NOte:
Optimal dilutions for each application to be determined by the researcher.