DACH2 Antibody

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ProSci
Product Code: PSI-46-650
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-46-650-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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46-650 (0.03ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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46-650 (0.03ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
DACH2, dachshund homolog 2 (Drosophila), RP11-345E19.1, FLJ31391, MGC138545, dachshund 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65-70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 65.3kDa according to human NP_444511.1 and of 68.6kDa according to mouse NP_291083.1). Recommended concentration: 0.03-0.1ug/ml.
Background References:
  • Wu K, Yang Y, Wang C, Davoli MA, D'Amico M, Li A, Cveklova K, Kozmik Z, Lisanti MP, Russell RG, Cvekl A, Pestell RG. DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. J Biol Chem. 2003 Dec 19;278(51):51673-84. Epub 2003 Oct 2.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-TRKQAVNSSRPGR
NCBI Gene ID #:
117154
NCBI Official Name:
dachshund family transcription factor 2
NCBI Official Symbol:
DACH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_444511.1
Protein GI Number:
16876441
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
.
Swissprot #:
Q96NX9