Leptin Antibody

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ProSci
Product Code: PSI-XP-5213Bt
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5213Bt-0.05mg0.05mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Leptin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze

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To detect Rat Leptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat Leptin.
2 / 3
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 3
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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To detect Rat Leptin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat Leptin.
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Further Information

Additional Names:
OB, obese, Ob, Leptin, Obesity factor,
Application Note:
ELISA:

Sandwich:
To detect Rat Leptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat Leptin (XP-5213) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat Leptin.

Western Blot:

To detect rat Leptin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat Leptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rat Leptin (rat Leptin).
NCBI Gene ID #:
25608
NCBI Official Name:
leptin
NCBI Official Symbol:
Lep
NCBI Organism:
Rattus norvegicustus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P50596
Protein GI Number:
1709436
Purification:
Anti-rat Leptin specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Signal Transduction,Growth Factor,Obesity,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P50596
User NOte:
Centrifuge vial prior to opening.