IL-3 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5185-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
IL-3 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
IL-3, MCGF, MULTI-CSFInterleukin-3, Hematopoietic growth factor, IL-3
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (1.0 ng/mL), a concentration of 0.38 - 0.57 μg/mL of this antibody is required.
ELISA:
To detect hIL-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Sandwich
To detect hIL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-3 (XP-5185Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:
To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-3 (1.0 ng/mL), a concentration of 0.38 - 0.57 μg/mL of this antibody is required.
ELISA:
To detect hIL-3 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Sandwich
To detect hIL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-3 (XP-5185Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-3.
Western Blot:
To detect hIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
The pleiotropic IL-3 (Interleukin 3) is a 15kDa cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-3 (human Interleukin-3).
NCBI Gene ID #:
3562
NCBI Official Name:
interleukin 3 (colony-stimulating factor, multiple)
NCBI Official Symbol:
IL3
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P08700
Protein GI Number:
124330
Purification:
Anti-hIL-3 specific antibody was purified by affinity chromatography employing immobilized hIL-3 matrix.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P08700
User NOte:
Centrifuge vial prior to opening.