IL-5 Antibody
| Code | Size | Price |
|---|
| PSI-XP-5191Bt-0.05mg | 0.05mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
IL-5 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-t
Documents
Further Information
Additional Names:
EDF, TRF, IL-5Interleukin-5, B-cell differentiation factor I, IL-5
Application Note:
ELISA:
Sandwich:
To detect hIL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-5 (XP-5191) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:
To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich:
To detect hIL-5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human IL-5 (XP-5191) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-5.
Western Blot:
To detect hIL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Interleukin 5 (IL-5) is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL-2, IL-4 and growth hormone. Human and mouse IL-5 have 70% amino acid sequence homology.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-5 (human IL-5).
NCBI Gene ID #:
3567
NCBI Official Name:
interleukin 5 (colony-stimulating factor, eosinophil)
NCBI Official Symbol:
IL5
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Protein Accession #:
P05113
Protein GI Number:
124341
Purification:
Anti-hIL-5 specific antibody was purified by affinity chromatography and then biotinylated.
Research Area:
Immunology,Chemokines & Cytokines,Antibody Pairs
Swissprot #:
P05113
User NOte:
Centrifuge vial prior to opening.