IP-10 Antibody

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ProSci
Product Code: PSI-XP-5204
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-XP-5204-0.1mg0.1mg£524.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Neutralisation
  • Western Blot (WB)
Storage:
IP-10 antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-

Images

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To detect mIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IP-10 (XP-5204Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.
2 / 5
To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
3 / 5
To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
4 / 5
This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
5 / 5
This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
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To detect mIP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine IP-10 (XP-5204Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.
To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
This antibody stained colchicine injected mouse brain (including the hippocampus region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

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Further Information

Additional Names:
C7, IP10, CRG-2, INP10, IP-10, Ifi10, mob-1, Scyb10, gIP-10, Crg2, Inp10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIP-10 (15.00 ng/mL), a concentration of 0.5 0.9 μg/mL of this antibody is required.

ELISA:

To detect mIP-10 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIP-10.

Sandwich:
br>To detect mIP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IP-10 (XP-5204Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIP-10.

Western Blot:

To detect mIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ng/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
Interferon-gamma-inducible 10 kD protein (IP-10), is a CXC chemokine with chemoattractant properties for CD4-positive T cells and inhibits early normal and leukemic hemopoietic progenitor proliferation. IP-10 is produced by a wide variety of cell types ranging from neutrophils and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV encephalitis, cutaneous T cell lymphoma, chronic hepatitis and acute anterior uveitis. Various observations strongly suggest a role for the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIP-10 (murine IP-10).
NCBI Gene ID #:
15945
NCBI Official Name:
chemokine (C-X-C motif) ligand 10
NCBI Official Symbol:
Cxcl10
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P17515
Protein GI Number:
124494
Purification:
Anti-mIP-10 specific antibody was purified by affinity chromatography employing immobilized mIP-10 matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P17515
User NOte:
Centrifuge vial prior to opening.