JE(MCP-1) Antibody
| Code | Size | Price |
|---|
| PSI-XP-5206-0.1mg | 0.1mg | £524.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Neutralisation
- Western Blot (WB)
Storage:
JE(MCP-1) antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated fre
-Antibody_E_XP-5206.png "To detect mJE(MCP-1) by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Murine JE(MCP-1)(XP-5206Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).")
-Antibody_WB_XP-5206.png "To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.")
-Antibody_WB-2_XP-5206.png "To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.")
-Antibody_IHC_XP-5206.jpg "This antibody stained colchicine injected mouse brain (including caudate putamen) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.")
Documents
Further Information
Additional Names:
JE, HC11, MCAF, MCP1, MCP-1, Scya2, Sigje, SMC-CF, AI323594, Je, Mcp1, C-C motif chemokine 2, Monocyte chemoattractant protein 1
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mJE (MCP-1) (100 ng/mL), a concentration of 4.0 - 6.0 μg/mL of this antibody is required.
ELISA:
To detect mJE (MCP-1) by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE (MCP-1).
Sandwich:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine JE(MCP-1)(XP-5206Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Western Blot:
To detect mJE (MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE (MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To yield one-half maximal inhibition [ND50] of the biological activity of mJE (MCP-1) (100 ng/mL), a concentration of 4.0 - 6.0 μg/mL of this antibody is required.
ELISA:
To detect mJE (MCP-1) by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mJE (MCP-1).
Sandwich:
To detect mJE(MCP-1) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine JE(MCP-1)(XP-5206Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Western Blot:
To detect mJE (MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE (MCP-1) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE (MCP-1) (murine JE (Macrophage/Monocyte chemotactic protein-1)).
NCBI Gene ID #:
20296
NCBI Official Name:
chemokine (C-C motif) ligand 2
NCBI Official Symbol:
Ccl2
NCBI Organism:
Mus musculus
Physical State:
Lyophilized
Protein Accession #:
P10148
Protein GI Number:
126844
Purification:
Anti-mJE (MCP-1) specific antibody was purified by affinity chromatography employing immobilized mJE (MCP-1) matrix.
Research Area:
Chemokines & Cytokines,Antibody Pairs, Functional Assays
Swissprot #:
P10148
User NOte:
Centrifuge vial prior to opening.