p53 Antibody
| Code | Size | Price |
|---|
| PSI-XPS-1021-0.1mL | 0.1mL | £686.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Monkey
Application: Western Blot (WB)
Storage:
For long term storage ?80˚C is recommended, but shorter term storage at ?20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Documents
Further Information
Application Note:
Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Background:
p53 has a well established role in the blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition p53 has also been linked to affects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. We raised this polyclonal antibody against a peptide representing the sequence around Ser392 of p53.
Background References:
- Saito, S., Yamaguchi, H., Higashimoto, Y., Chao, C., Xu, Y., Fornace, A.J., Jr., Appella, E., and Anderson, C.W., "Phosphorylation site interdependence of human p53 post-translational modifications in response to stress," J. Biol. Chem., 278 (2003) 37,536-37,544.
- Sharpless,N.E. and DePinho,R.A., "p53: Good cop/bad cop," Cell, 110 (2002) 9-12.
- Jiang, Y.H., Armstrong, D., Albrecht, U., Atkins, C.M., Noebels, J.L., Eichele, G., Sweatt, J.D., and Beaudet, A.L., "Mutation of the angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation," Neuron, 21 (1998) 799 - 811.
- Pise-Masison, C.A., Radonovich, M., Sakaguchi, K., Appella, E., and Brady, J.N., "Phosphorylation of p53: a novel pathway for p53 inactivation in human T- cell lymphotropic virus type 1-transformed cells," J. Virol., 72 (1998) 6348-6355.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
p53 (Ser392) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53.
Modifications:
Phospho-Specific
NCBI Gene ID #:
7157
NCBI Official Name:
tumor protein p53
NCBI Official Symbol:
TP53
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53
Protein Accession #:
P04637
Protein GI Number:
269849759
Purification:
Affinity Purified
Research Area:
Apoptosis ,Phospho-Specific
SPECIFICITY:
p53 antibody is specific for the 53 kDa p53 protein phosphorylated at Ser392.
Swissprot #:
P04637
User NOte:
Optimal dilutions for each application to be determined by the researcher.