ADH1 Antibody

Contact us
ProSci
Product Code: PSI-45-004
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-004-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

1 / 1
45-004 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
❮❯

45-004 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
pan ADH, ADH1B, ADH2, alcohol dehydrogenase IB (class I), beta polypeptide, ADH, beta subunit, aldehyde reductase, alcohol dehydrogenase 2, alcohol, ADH, gamma subunit, alcohol dehydrogenase 3 (class I), gamma polypeptide dehydrogenase 2 (class I), beta polypeptide, alcohol dehydrogenase 1B (class I), beta polypeptide, ADH1C, alcohol dehydrogenase 1C (class I), gamma polypeptide, ADH3, aldehyde reductase, class I alcohol dehydrogenase, gamma subunit, ADH1A, alcohol dehydrogenase 1A (class I), alpha polypeptide, ADH1, ADH, alpha subunit, alcohol dehydrogenase 1 (class I), alpha polypeptide, aldehyde reductase, class I alcohol dehydrogenase, alpha subunit
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 38kDa band observed in Human Liver lysates (calculated MW of 39.9kDa according to NP_000658.1, NP_000659.2 and 000660.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Customer found this product to work in IHC on Human Liver.
Background References:
  • Ikuta T, Fujiyoshi T, Kurachi K, Yoshida A. Molecular cloning of a full-length cDNA for human alcohol dehydrogenase. Proc Natl Acad Sci USA. 1985 May;82(9):2703-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: STAGKVMKCKA
NCBI Gene ID #:
124; 125; 126
NCBI Official Name:
alcohol dehydrogenase 1A (class I), alpha polypeptide
NCBI Official Symbol:
ADH1A, B, C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_000658.1, NP_000659.2, NP_000660.1
Protein GI Number:
4501929
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognise the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of human alcohol dehydrogenase.
Swissprot #:
P07327