Androgen Receptor Antibody

Product Code: PSI-45-010

Supplier: ProSci

Code	Size	Price

PSI-45-010-0.1mg

0.1mg

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Western Blot (WB)

Storage:

Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-010(0.5ug/ml) staining of Human Cerebellum (A) and (0.1ug/ml) negative control Pancreas (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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45-010 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear st

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45-010 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial/cytoplasmic staining. The nuclear st

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Documents

Further Information

Additional Names:

AR, androgen receptor, dihydrotestosterone receptor, KD, AIS, TFM, DHTR, SBMA, NR3C4, SMAX1, HUMARA, HYSP1, androgen receptor, dihydrotestosterone receptor, RP11-383C12.1

Application Note:

Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in Human Cerebellum lysates (calculated MW of 99.2kDa according to NP_000035.2). Recommended concentration:0.1-0.5ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 2-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the mitochondria/cytoplasm of MCF7 and U2OS cells. Recommended concentration: 10ug/ml.

Chromatin Immunoprecipitation: A six-fold increased presence of the FKBP5 locus observed when comparing the precipitations using EB06441 with non-specific goat IgG to bring down Androgen Receptor in a chromatin lysate from DHT-stimulated HEC50 cells.

Background References:

Trapman J, Klaassen P, Kuiper GG, van der Korput JA, Faber PW, van Rooij HC, Geurts van Kessel A, Voorhorst MM, Mulder E, Brinkmann AO. Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 1988 May 31;153(1):241-8.

Buffer:

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.

Concentration:

500 ug/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow

Immunogen:

The immunogen for this antibody is: EVQLGLGRVYPRPPSC

NCBI Gene ID #:

367

NCBI Official Name:

androgen receptor

NCBI Official Symbol:

NCBI Organism:

Homo sapiens

Physical State:

Liquid

Protein Accession #:

NP_000035.2

Protein GI Number:

21322252

Purification:

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Research Area:

Homeostasis

SPECIFICITY:

This antibody is expected to recognize isoform 1 (NP_000035.2) only.

Swissprot #:

P10275