Annexin Antibody

ProSci
Product Code: PSI-45-008
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-008-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-008 staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

45-008 staining (0.5ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Further Information

Additional Names:
ANXA2, Annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, HEL-S-270, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calpactin I heavy polypeptide, chromobindin 8, lipocortin II, placental anticoagulant protein IV
Application Note:
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 35kDa band observed in lysates of cell lines A549, HeLa and NIH3T3 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002857.1). Recommended concentration: 0.5-3ug/ml.
Background References:
  • Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD. Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption. J Biol Chem 1994 Nov 18;269(46):28696-701
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Cow, Pig
Immunogen:
The immunogen for this antibody is: STVHEILCKLSLEGD
NCBI Gene ID #:
302
NCBI Official Name:
annexin A2
NCBI Official Symbol:
ANXA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001002858.1, NP_004030.1
Protein GI Number:
50845388
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis ,Signal Transduction
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_001002858.1; NP_004030.1). Variants NP_004030.1, NP_001002857.1 and NP_001129487.1 represent identical protein.
Swissprot #:
P07355