CKM Antibody

ProSci
Product Code: PSI-25-702
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-25-702-100ul100ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CKM antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human Heart at 0.2-1 ug/ml.

Antibody used in WB on Human Heart at 0.2-1 ug/ml.

Further Information

Additional Names:
CKM, CKMM, M-CK
Application Note:
CKM antibody can be used for detection of CKM by ELISA at 1:62500. CKM antibody can be used for detection of CKM by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
CKM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CKM is a member of the ATP: guanido phosphotransferase protein family.The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background References:
  • Yamin, C., (2007) J. Appl. Physiol. 103 (6), 2057-2061.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CKM.
NCBI Gene ID #:
1158
NCBI Official Name:
creatine kinase, muscle
NCBI Official Symbol:
CKM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
NP_001815
Protein GI Number:
21536288
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
P06732
User NOte:
Optimal dilutions for each application to be determined by the researcher.