DPH2 Antibody

ProSci
Product Code: PSI-26-626
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-26-626-100ul100ul£662.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store DPH2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on Human 293T at 0.2-1 ug/ml.

Antibody used in WB on Human 293T at 0.2-1 ug/ml.

Further Information

Additional Names:
DPH2, DPH2L2
Application Note:
DPH2 antibody can be used for detection of DPH2 by ELISA at 1:62500. DPH2 antibody can be used for detection of DPH2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
DPH2 gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Liu, S., (2004) Mol. Cell. Biol. 24 (21), 9487-9497.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DPH2.
NCBI Gene ID #:
1802
NCBI Official Name:
DPH2 homolog (S. cerevisiae)
NCBI Official Symbol:
DPH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
NP_001375
Protein GI Number:
21536476
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q9BQC3
User NOte:
Optimal dilutions for each application to be determined by the researcher.