EIF5 Antibody
| Code | Size | Price |
|---|
| PSI-26-794-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
- Rat
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
EIF5, EIF-5A, EIF-5
Application Note:
EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Background References:
- Olsen, J.V., (2006) Cell 127 (3), 635-648.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF5.
NCBI Gene ID #:
1983
NCBI Official Name:
eukaryotic translation initiation factor 5
NCBI Official Symbol:
EIF5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
NP_892116
Protein GI Number:
37537716
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
P55010
User NOte:
Optimal dilutions for each application to be determined by the researcher.