H1FOO Antibody
| Code | Size | Price |
|---|
| PSI-31-305-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store H1FOO antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
H1FOO, MGC50807, osH1
Application Note:
H1FOO antibody can be used for detection of H1FOO by ELISA at 1:312500. H1FOO antibody can be used for detection of H1FOO by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
H1FOO may play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. H1FOO is essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a member of the histone H1 family. This gene contains introns, unlike most histone genes. The protein encoded is a member of the histone H1 family. The related mouse gene is expressed only in oocytes.
Background References:
- Tanaka, M., (2005) Biol. Reprod. 72 (1), 135-142?.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human H1FOO.
NCBI Gene ID #:
132243
NCBI Official Name:
H1 histone family, member O, oocyte-specific
NCBI Official Symbol:
H1FOO
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
NP_722575
Protein GI Number:
24475863
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q8IZA3
User NOte:
Optimal dilutions for each application to be determined by the researcher.