OGDH Antibody
| Code | Size | Price |
|---|
| PSI-27-204-100ul | 100ul | £662.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store OGDH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Documents
Further Information
Additional Names:
OGDH, AKGDH, E1k, OGDC
Application Note:
OGDH antibody can be used for detection of OGDH by ELISA at 1:12500. OGDH antibody can be used for detection of OGDH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO (2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia.
Background References:
- van (2007) Am. J. Med. Genet. A 143 (7), 763-767.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human OGDH.
NCBI Gene ID #:
4967
NCBI Official Name:
oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
NCBI Official Symbol:
OGDH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
NP_001003941
Protein GI Number:
51873038
Purification:
Antibody is purified by peptide affinity chromatography method.
Swissprot #:
Q96DD3
User NOte:
Optimal dilutions for each application to be determined by the researcher.