UBE2N Antibody

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ProSci
Product Code: PSI-27-998
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-27-998-100ul100ul£609.00
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Overview

Host Type: Rabbit
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Canine (dog)
  • Fish
  • Fly
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
For short periods of storage (days) store at 4˚C. For longer periods of storage, store UBE2N antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Images

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Antibody used in WB on r-UBE2N at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
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Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.
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Antibody used in WB on r-UBE2N at: 1:500 (Lanes: 1: 40ng HIS-UBE2D1 protein, 2: 40ng HIS-UBE2D2 protein, 3: 40ng HIS-UBE2D3 proteinm, 4: 40ng HIS-UBE2D4 protein, 5: 40ng HIS-UBE2E1 protein, 6: 40ng HIS-UBE2E2 protein, 7: 40ng HIS-UBE2E3 protein, 8: 40ng HIS-UBE2K protein, 9: 40ng HIS-UBE2L3 protein, 10: 40ng HIS-UBE2N protein, 11: 40ng HIS-UBE2V1 protein, 12: 40ng HIS-UBE2V2 protein.).
Antibody used in WB on Human HepG2 cells at 1.25 ug/ml.

Documents

Further Information

Additional Names:
UBE2N, UBC13, UbcH13, HEL-S-71, UbcH-ben
Application Note:
UBE2N antibody can be used for detection of UBE2N by ELISA at 1:312500. UBE2N antibody can be used for detection of UBE2N by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
UBE2N encodes a member of the E2 ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Studies in mouse suggest that this protein plays a role in DNA postreplication repair.
Background References:
  • Bothos, J., et al., (2003) Oncogene 22 (46), 7101-7107.
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2N.
NCBI Gene ID #:
7334
NCBI Official Name:
ubiquitin-conjugating enzyme E2N
NCBI Official Symbol:
UBE2N
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
NP_003339
Protein GI Number:
4507793
Purification:
Antibody is purified by protein A chromatography method.
Swissprot #:
P61088
User NOte:
Optimal dilutions for each application to be determined by the researcher.