AP-2 gamma Antibody
| Code | Size | Price |
|---|
| PSI-45-259-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
TFAP2C, ERF1, TFAP2G, hAP-2g, AP2-GAMMA, transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma), estrogen receptor factor 1, transcription factor AP-2 gamma (activating enhancer-binding protein 2 gamma)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:WB experiments gave bands at approx 75kDa and 22kDa in Human Placenta and Ovary lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 49.2kDa according to NP_003213.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 2-3ug/ml.
Background References:
- Hoei-Hansen CE, Rajpert-De Meyts E, Carlsen E, Almstrup K, Leffers H, Skakkebaek NE. Fast-track Article: A subfertile patient diagnosed with testicular carcinoma in situ by immunocytological staining for AP-2{gamma} in semen samples: Case report. Hum Reprod. 2005 Jan 13;
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-NKTLEKMEKHRK
NCBI Gene ID #:
7022
NCBI Official Name:
transcription factor AP-2 gamma (activating enhancer binding protein 2 gamma)
NCBI Official Symbol:
TFAP2C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003213.1
Protein GI Number:
4507445
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q92754