APEX1 Antibody
| Code | Size | Price |
|---|
| PSI-45-009-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
APE1, APEX1, APEX, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, apurinic/apyrimidinic (abasic), APEX nuclease 1 endonuclease, deoxyribonuclease (apurinic or apyrimidinic), apurinic/apyrimidinic (abasic) endonuclease, redox factor 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in nuclear lysates of cell lines A431, HeLa and MCF7 (calculated MW of 35.6kDa according to NP_001632.2, NP_542379.1 and NP_542380.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Breast shows nuclear staining of lobular epithelial cells. Recommended concentration: 4-6ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Background References:
- Demple B, Herman T, Chen DS. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc Natl Acad Sci USA. 1991 Dec 15;88(24):11450-4.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: PKRGKKGAVAEDGD-C
NCBI Gene ID #:
328
NCBI Official Name:
APEX nuclease (multifunctional DNA repair enzyme) 1
NCBI Official Symbol:
APEX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001632.2
Protein GI Number:
18375501
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).
Swissprot #:
P27695