ARHGEF4 Antibody

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ProSci
Product Code: PSI-45-281
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-281-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-281 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-281 (2ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARHGEF4, Rho guanine nucleotide exchange factor (GEF) 4, ASEF, GEF4, STM6, KIAA1112, DKFZp434G2016, APC-stimulated guanine nucleotide exchange factor, ASEF1, Rho guanine nucleotide exchange factor 4
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Human Brain lysates and in transfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.1kDa according to NP_056135.2 and 76.4kDa according to 127462.1). Recommended concentration: 2-4ug/ml.
Background References:
  • Thiesen S, Kubart S, Ropers HH, Nothwang HG. Isolation of two novel human RhoGEFs, ARHGEF3 and ARHGEF4, in 3p13-21 and 2q22. Biochem Biophys Res Commun. 2000 Jun 24;273(1):364-9.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: PWEEPAGEKPSCS
NCBI Gene ID #:
50649
NCBI Official Name:
Rho guanine nucleotide exchange factor (GEF) 4
NCBI Official Symbol:
ARHGEF4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_056135.2, NP_127462.1
Protein GI Number:
15011979
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise both reported isoforms (NP_056135.2; NP_127462.1).
Swissprot #:
Q9NR80