ARIH1 Antibody

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ProSci
Product Code: PSI-45-282
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-282-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-282 staining (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
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45-282 staining (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARIH1, HHARI, ARI, HARI, UBCH7BP, ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1 (Drosophila), ariadne homolog, ariadne, Drosophila, homolog of, DKFZp686O13120, FLJ20329, FLJ93118, ariadne ubiquitin-conjugating enzyme E2 binding protein homolog 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band seen in U937 lysate [Predicted MW of approx. 61kDa according to NP_005735]. Recommended for use at 0.2-1ug/ml.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Background References:
  • Moynihan TP, Ardley HC, Nuber U, Rose SA, Jones PF, Markham AF, Scheffner M, Robinson PA. The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 1999 Oct 22;274(43):30963-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Cow
Immunogen:
The immunogen for this antibody is: C-HEGYEKDLWEYIED
NCBI Gene ID #:
25820
NCBI Official Name:
ariadne RBR E3 ubiquitin protein ligase 1
NCBI Official Symbol:
ARIH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005735.2
Protein GI Number:
187761373
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9Y4X5