ARIH2 Antibody

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ProSci
Product Code: PSI-45-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-283-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunoprecipitation (IP)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-283 (2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-283 (2ug/ml) staining of Hela (A) and K562 (B) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ARIH2, ariadne homolog 2 (Drosophila), ARI2, TRIAD1, all-trans retinoic acid inducible RING finger, ariadne-2 (D. melanogaster) homolog (all-trans retinoic acid inducible RING finger), FLJ10938, FLJ33921, OTTHUMP00000210388, ariadne homolog 2
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 60kDa band observed in nuclear lysates of Hela and K562 (Calculated MW of 57.8kDa according to NP_006312.1) Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Customer's experiments gave a band at approx 60kDa in lysates in both Human HeLa and Mouse Muscle (IM2 & C2C12) cells. This band disappeared after RNAi knock-down. Calculated MW of 57.8kDa according to NP_006312.1). Recommended concentration: 1-3ug/ml. This antibody has also been successfully used in WB in PMID: 24486325.Immunofluorescence: Customer's experiments showed nuclear staining in DAPI with IF. This antibody has also been successfully used in IF in PMID: 24486325.
Background References:
  • van der Reijden BA, Erpelinck-Verschueren CA, Lowenberg B, Jansen JH. TRIADs: a new class of proteins with a novel cysteine-rich signature. Protein Sci. 1999 Jul;8(7):1557-61.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EQRRRTLLKDFHDT
NCBI Gene ID #:
10425
NCBI Official Name:
ariadne RBR E3 ubiquitin protein ligase 2
NCBI Official Symbol:
ARIH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006312.1
Protein GI Number:
5453557
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
O95376