ASF1A Antibody

ProSci
Product Code: PSI-45-301
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-301-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-301 (1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-301 (1ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
ASF1A, ASF1 anti-silencing function 1 homolog A (S. cerevisiae), RP3-329L24.1, CGI-98, CIA, DKFZP547E2110, HSPC146, ASF1 anti-silencing function 1 homolog A, CCG1-interacting factor A, anti-silencing function 1A
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 21kDa band observed in lysates of cell line Jurkat (calculated MW of 23.0kDa according to NP_054753.1). Recommended concentration: 1-3ug/ml.
Background References:
  • Groth A, Ray-Gallet D, Quivy JP, Lukas J, Bartek J, Almouzni G. Human Asf1 regulates the flow of S phase histones during replicational stress. Mol Cell. 2005 Jan 21;17(2):301-11.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: C-ENSLNVMLESH
NCBI Gene ID #:
25842
NCBI Official Name:
anti-silencing function 1A histone chaperone
NCBI Official Symbol:
ASF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_054753.1
Protein GI Number:
7661592
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9Y294