ATAD5 Antibody

ProSci
Product Code: PSI-45-303
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-303-0.1mg0.1mg£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Further Information

Additional Names:
Atad5, ATPase family, AAA domain containing 5, C17orf41, chromosome 17 open reading frame 41, FLJ12735, FRAG1 , chromosome fragility associated gene 1, hypothetical protein LOC237877, RP23-328O2.1, C130052G03Rik
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx 60kDa band in lysates of cell lines NIH3T3 and NSO after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 204kDa according to NP_001025027.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Ishii H, Inageta T, Mimori K, Saito T, Sasaki H, Isobe M, Mori M, Croce CM, Huebner K, Ozawa K, Furukawa Y. Frag1, a homolog of alternative replication factor C subunits, links replication stress surveillance with apoptosis. Proc Natl Acad Sci USA. 2005 Jul 5;102(27):9655-60. Epub 2005 Jun 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-QSKDQPLRKSQK
NCBI Gene ID #:
237877 (mouse); 303348 (rat);
NCBI Official Name:
ATPase family, AAA domain containing 5
NCBI Official Symbol:
Atad5
NCBI Organism:
Mus musculus
Physical State:
Liquid
Protein Accession #:
NP_001025027.1
Protein GI Number:
71143102
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
Swissprot #:
Q4QY64