ATF2 Antibody

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ProSci
Product Code: PSI-45-012
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-012-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-012 (1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-012 (1ug/ml) staining of HepG2 (A), K562 (B) and HeLa (C) nuclear lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558, activating transcription factor 2 splice variant ATF2-var2, cAMP responsive element binding protein 2, formerly
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa observed in nuclear lysates of cell lines HeLa, HepG2 and K562, which corresponds with different antibodies from other commercial sources. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Background References:
  • Monzen K, Hiroi Y, Kudoh S, Akazawa H, Oka T, Takimoto E, Hayashi D, Hosoda T, Kawabata M, Miyazono K, Ishii S, Yazaki Y, Nagai R, Komuro I. Smads, TAK1, and their common target ATF-2 play a critical role in cardiomyocyte differentiation. J Cell Biol 2001 May 14;153(4):687-98
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-IVMAPSSQSQPSGS
NCBI Gene ID #:
1386
NCBI Official Name:
activating transcription factor 2
NCBI Official Symbol:
ATF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001871.2
Protein GI Number:
22538422
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction ,p53 Network ,Cancer
SPECIFICITY:
.
Swissprot #:
P15336