ATG5 Antibody
| Code | Size | Price |
|---|
| PSI-46-954-0.1mg | 0.1mg | £505.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
Documents
Further Information
Additional Names:
ATG5, ATG5 autophagy related 5 homolog (S. cerevisiae), APG5, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000040507, apoptosis specific protein
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa according to NP_004840.1. The 38 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
- Lee YR, Lei HY, Liu MT, Wang JR, Chen SH, Jiang-Shieh YF, Lin YS, Yeh TM, Liu CC, Liu HS. Autophagic machinery activated by dengue virus enhances virus replication. Virology. 2008 May 10;374(2):240-8. Epub 2008 Mar 18.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Mouse, Cow, Horse
Immunogen:
The immunogen for this antibody is: C-SQVINEMQKKDHKQ
NCBI Gene ID #:
9474
NCBI Official Name:
autophagy related 5
NCBI Official Symbol:
ATG5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_004840.1
Protein GI Number:
4757798
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Autophagy
Swissprot #:
Q9H1Y0