Aurora Kinase Antibody

ProSci
Product Code: PSI-45-014
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-014-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-014 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-014 (0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, serine/threonine protein kinase 15, serine/threonine protein kinase 6, OTTHUMP00000031340, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). Recommended concentration: 0.1-0.3ug/ml.Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from a customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Background References:
  • Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 1998 Oct;20(2):189-93
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-QNKESASKQS
NCBI Gene ID #:
6790
NCBI Official Name:
aurora kinase A
NCBI Official Symbol:
AURKA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003591.2
Protein GI Number:
38327562
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Signal Transduction
SPECIFICITY:
Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1
Swissprot #:
O14965