BAALC Antibody

ProSci
Product Code: PSI-45-313
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-313-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-313 (0.03ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-313 (0.03ug/ml) staining of mouse brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
BAALC, brain and acute leukemia, cytoplasmic , HGNC:14333, FLJ12015
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 18kDa band observed in mouse brain lysates (calculated MW of 15.6kDa according to human NP_079088.1 and of 15.5kDa according to mouse NP_542371.1. Recommended concentration: 0.03-0.1ug/ml. An approx, 48kDa band was consistently observed in both Human and Mouse Brain lysates. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A. Baalc, a marker of mesoderm and muscle. Gene Expr Patterns. 2005 Apr;5(4):463-73.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Rat, Pig
Immunogen:
The immunogen for this antibody is: C-KQNGLQTTEAKRDAK
NCBI Gene ID #:
79870
NCBI Official Name:
brain and acute leukemia, cytoplasmic
NCBI Official Symbol:
BAALC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079088.1
Protein GI Number:
13376200
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise isoform 1 (NP_079088.1) only.
Swissprot #:
Q8WXS3