BCAR3 Antibody

ProSci
Product Code: PSI-45-324
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-324-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-324 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-324 (0.5ug/ml) staining of Mouse Kidney epithelial cells lysate (15ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
BCAR3, NSP2, SH2D3B, breast cancer anti-estrogen resistance 3, KIAA0554, OTTHUMP00000011961, breast cancer antiestrogen resistance 3, dJ1033H22.2 (breast cancer anti-estrogen resistance 3)
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Mouse embryonic Kidney epithelial cell lysates (calculated MW of 92.6kDa according to Human NP_003558.1) and 92.3kDa according to Mouse NP_038895.1. Recommended concentration: 0.5-2ug/ml. Data kindly provided by anonymous customer.
Background References:
  • van Agthoven T, van Agthoven TL, Dekker A, van der Spek PJ, Vreede L, Dorssers LC. Identification of BCAR3 by a random search for genes involved in antiestrogen resistance of human breast cancer cells. EMBO J. 1998 May 15;17(10):2799-808.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RKLEPPPVKQAEL
NCBI Gene ID #:
8412
NCBI Official Name:
breast cancer anti-estrogen resistance 3
NCBI Official Symbol:
BCAR3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003558.1
Protein GI Number:
4502371
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
O75815