BERP Antibody

ProSci
Product Code: PSI-45-327
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-327-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-327 staining (0.05ug/ml) of Mouse Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
2 / 2

45-327 staining (0.05ug/ml) of Mouse Brain lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
TRIM3, BERP, RNF22, TRIM3, tripartite motif-containing 3, HAC1, RNF97, ring finger protein 22, brain expressed ring finger, tripartite motif protein TRIM3, FLJ16135
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 80kDa band observed in Human Brain (Cerebellum) and Rat Brain lysates and approx 85kDa band seen in Mouse Brain (calculated MW of 80.8kDa according to Human NP_006449.2, Mouse NP_001272799.1 and Rat NP_113974.1). Recommended concentration: 0.1-0.3ug/ml.
Background References:
  • El-Husseini AE, Fretier P, Vincent SR. Cloning and characterization of a gene (RNF22) encoding a novel brain expressed ring finger protein (BERP) that maps to human chromosome 11p15.5. Genomics. 2001 Feb 1;71(3):363-7.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: AKREDSPGPEVQP-C
NCBI Gene ID #:
10612
NCBI Official Name:
tripartite motif containing 3
NCBI Official Symbol:
TRIM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006449.2, NP_150594.2
Protein GI Number:
32454739
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
Both variants (NP_006449.2; NP_150594.2;) represent the same protein. This antibody is expected to recognise all three human isoforms of this protein.
Swissprot #:
O75382