BIM Antibody

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ProSci
Product Code: PSI-45-330
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-330-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-330 staining (0.5ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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45-330 staining (0.5ug/ml) of K562 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Documents

Further Information

Additional Names:
BCL2L11, BCL2-like 11 (apoptosis facilitator), BAM, BIM, BOD, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, bcl-2-like protein 11, bcl-2-related ovarian death agonist, bcl2-L-11, Bim-alpha1, Bim-alpha2, Bim-alpha3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 23kDa band observed in K562 lysates (predicted MW of 18.5kDa according to NP_619528.1). Recommended for use at 0.5-2ug/ml.
Background References:
  • Marani M, Tenev T, Hancock D, Downward J, Lemoine NR. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol. 2002 Jun;22(11):3577-89.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-FNAYYARRLEK
NCBI Gene ID #:
10018
NCBI Official Name:
BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol:
BCL2L11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_619528.1, NP_619529.1, NP_996886.1,
Protein GI Number:
20336317
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).
Swissprot #:
O43521