BNIP1 Antibody

ProSci
Product Code: PSI-45-335
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-335-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-335 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

45-335 (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Further Information

Additional Names:
BNIP1, BCL2/adenovirus E1B 19kDa interacting protein 1, NIP1, SEC20, TRG-8, BCL2/adenovirus E1B 19kD interacting protein 1, BCL2/adenovirus E1B 19kD-interacting protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Kidney lysates (calculated MW of 26.1kDa according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Nakajima K, Hirose H, Taniguchi M, Kurashina H, Arasaki K, Nagahama M, Tani K, Yamamoto A, Tagaya M. Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. EMBO J. 2004 Aug 18;23(16):3216-26. Epub 2004 Jul 22.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-DLLRQRKTTKESLAQ
NCBI Gene ID #:
662
NCBI Official Name:
BCL2/adenovirus E1B 19kDa interacting protein 1
NCBI Official Symbol:
BNIP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001196.1, NP_053581.1, NP_053582.1, NP_053583.1
Protein GI Number:
4502441
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
All reported variants represent isoform 1 (NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1)
Swissprot #:
Q12981