CARD11 Antibody
Code | Size | Price |
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PSI-45-360-0.1mg | 0.1mg | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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Further Information
Additional Names:
CARD11, caspase recruitment domain family, member 11, BIMP3, CARMA1, MGC133069, card-maguk protein 1, bcl10-interacting maguk protein 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150+65kDa bands observed in lysates of cell line Jurkat (calculated MW of 133kDa according to NP_115791.2). Recommended concentration: 1-3ug/ml.
An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:In paraffin embedded Human Spleen shows nuclear staining. Recommended concentration: 2-5ug/ml.
Background References:
- Bertin J, Wang L, Guo Y, Jacobson MD, Poyet JL, Srinivasula SM, Merriam S, DiStefano PS, Alnemri ES. CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B. J Biol Chem. 2001 Apr 13;276(15):11877-82. Epub 2001 Jan 12.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QRKTIWVDEDQL
NCBI Gene ID #:
84433
NCBI Official Name:
caspase recruitment domain family, member 11
NCBI Official Symbol:
CARD11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_115791.3
Protein GI Number:
16507952
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Infectious Disease
SPECIFICITY:
.
Swissprot #:
Q9BXL7