CBR1 Antibody

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ProSci
Product Code: PSI-45-368
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-368-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-368 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-368 (0.1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-368 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-368 (0.1ug/ml) staining of U251 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CBR1, carbonyl reductase 1, CBR, carbonyl reductase (NADPH), carbonyl reductase (NADPH) 1, hCBR1, prostaglandin 9-ketoreductase, prostaglandin-E(2) 9-reductase, SDR21C1, short chain dehydrogenase/reductase family 21C, member 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 30-35kDa band observed in lysates of Glioblastoma cell line U251 and in Human Kidney lysates (calculated MW of 30.4kDa according to NP_001748.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Kidney shows cytoplasmic staining of epithelial cells of renal tubules. Recommended concentration: 1-2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10ug/ml.
Background References:
  • Balcz B, Kirchner L, Cairns N, Fountoulakis M, Lubec G Increased brain protein levels of carbonyl reductase and alcohol dehydrogenase in Down syndrome and Alzheimer's disease. J Neural Transm Suppl. 2001;(61):193-201
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-HGQFVSEKRVEQW
NCBI Gene ID #:
873
NCBI Official Name:
carbonyl reductase 1
NCBI Official Symbol:
CBR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001748.1
Protein GI Number:
4502599
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
P16152