CBX8 Antibody

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ProSci
Product Code: PSI-45-374
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-45-374-0.1mg0.1mg£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Caprine (Goat)
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.

Images

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45-374 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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45-374 (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Documents

Further Information

Additional Names:
CBX8, chromobox homolog 8 (Pc class homolog, Drosophila) , HPC3, PC3, RC1, chromobox homolog 8, polycomb 3
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 40kDa band observed in lysates of cell line K562 (calculated MW of 43.4kDa according to NP_065700.1). Recommended concentration: 0.1-0.3ug/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Background References:
  • Dietrich N, Bracken AP, Trinh E, Schjerling CK, Koseki H, Rappsilber J, Helin K, Hansen KH. Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus. EMBO J. 2007 Mar 21;26(6):1637-48. Epub 2007 Mar 1.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-RERGTSRVDDKPSS
NCBI Gene ID #:
57332
NCBI Official Name:
chromobox homolog 8
NCBI Official Symbol:
CBX8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065700.1
Protein GI Number:
10190682
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q9HC52